Title: Sonochemical-solvothermal synthesis of guanine embedded copper based metal-organic framework (MOF) and its effect on oprD gene expression in clinical and standard strains of Pseudomonas aeruginosa
Authors: Abbasloo, F (Abbasloo, Farideh); Khosravani, SA (Khosravani, Seyed Abdolmajid); Ghaedi, M (Ghaedi, Mehrorang); Dashtian, K (Dashtian, Kheibar); Hosseini, E (Hosseini, Ebrahim); Manzouri, L (Manzouri, Leila); Khorramrooz, SS (Khorramrooz, Seyed Sajjad); Sharifi, A (Sharifi, Asghar); Jannesar, R (Jannesar, Ramin); Sadri, F (Sadri, Farzad)
The guanine incropped Cu based metal-organic framework (Guanine-Cu-MOF) was synthesized by facile one-step sonochemical method by simply mixing of 4-4, biphenyldicarboxylic, guanine and copper nitrate (Bio-Cu-H(2)bpdc-Gu). The prepared guanine-MOF was characterized by using X-Ray diffraction (XRD), Fourier-transform infrared spectroscopy (FFIR), and Field emission scanning electron microscopy (FE-SEM) techniques. The morphology of prepared material was sponge-shaped which it was well documented, together with the presence of existing functional groups. The effect of prepared material on oprD Gene Expression was investigated in Clinical and Standard Strains of Pseudomonas aeruginosa (PAO-1) and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of prepared samples against P. aeruginosa strains were determined through the broth micro-dilution method. The expression of oprD gene in strains affected by Cu-H(2)bpdc-Gu was quantitatively investigated through real-time PCR. MIC of Bio-Cu-H(2)bpdc-Gu was 400 mu g/mL for the standard and clinical strains of P. aeruginosa, while, MBC of this compound was 700 mu g/mL for standard strain and 800 mu g/mL for clinical strains. The highest and the lowest rate of oprD gene expression were found to be 3.6 and 1.1 fold in the strains, respectively.
Title: Ultrasound-accelerated synthesis of gold nanoparticles modified choline chloride functionalized graphene oxide as a novel sensitive bioelectrochemical sensor: Optimized meloxicam detection using CCD-RSM design and application for human plasma sample
Authors: Bahrani, S (Bahrani, Sonia); Razmi, Z (Razmi, Zahra); Ghaedi, M (Ghaedi, Mehrorang); Asfaram, A (Asfaram, Arash); Javadian, H (Javadian, Hamedreza)
In this research, gold nanoparticles modified choline chloride functionalized graphene oxide (AuNPs-ChCl-GO) was synthesized through the assistance of ultrasound and fabricated as a novel bioelectrochemical sensor and utilized for the sensitive detection of meloxicam (MEL). The morphological and structural features of the AuNPs-ChCl-GO were characterized using different techniques including FTIR, TEM, FE-SEM, EDX, and XRD. The modified electrode showed a remarkable improvement in the anodic oxidation activity of MEL due to the enhancement in the current response compared to the bare carbon paste electrode (CPE). The biosensor composition and measurement conditions were optimized using an experimental design. The differential pulse voltammetry (DPVs) exhibited expanded linear dynamic in the range of 9.0 x 10(-9) to 8.5 x 10(-7) M for MEL in Britton-Robinson buffer at pH = 4.0 with a detection limit of 1.008 x 10(-9) M. The practical utility of the modified electrode was demonstrated by the accurate detection of MEL in human plasma sample.
Title: Diazinon Interrupts Ovarian Steroidogenic Acute Regulatory (StAR) Gene Transcription in Gonadotropin-Stimulated Rat Model
Authors: Siavashpour, A (Siavashpour, Asma); Ghasemi, Y (Ghasemi, Younes); Khalvati, B (Khalvati, Bahman); Jeivad, F (Jeivad, Fereshteh); Azarpira, N (Azarpira, Negar); Niknahad, H (Niknahad, Hossein)
Organophosphate pesticides are considered as endocrine disruptors that interfere with reproductive functions. The corpus luteum (CL) is a transient endocrine gland that produces progesterone, a crucial hormone for a successful beginning and maintenance of pregnancy. Steroidogenic acute regulatory protein (StAR) facilitates the rate-limiting transfer of cholesterol from the outer mitochondrial membrane to the inner organelle membranes. We investigated the effect of Diazinon (DZN), an organophosphate, on StAR mRNA expression by Sybergreen Real Time-PCR in a time-dependent manner in luteal phase. Fifty immature female Wistar rats (24-day-old) were injected with a single injection of Pregnant mare's Serum Gonadotropin (PMSG) followed by a single injection of human Chorionic Gonadotropin (hCG), 48 h later. Then, DZN was administered in a single dose (70 mg/kg bw, I. P), controls received only the vehicle, 12 h post-hCG injection. Ovaries were collected in 4 h. intervals from 8 to 24 h post-hCG injection. Then, hCG stimulation transcript levels of StAR gene were significantly altered in the hormone-stimulated rats following DZN treatment. In addition, histological study showed that the CL diameter in DZN-treated group was smaller than control group (p = 0.000). Our findings suggest that the critical step in the function of CL is disrupted by DZN and may correlates with female reproductive damage.
Title: Magnetic based nanocomposite sorbent combination with ultrasound assisted for solid-phase microextraction of Azure II in water samples prior to its determination spectrophotometric
Authors: Dastkhoon, M (Dastkhoon, Mehdi); Ghaedi, M (Ghaedi, Mehrorang); Asfaram, A (Asfaram, Arash); Jannesar, R (Jannesar, Ramin); Sadeghfar, F (Sadeghfar, Fardin)
Present report designed for monitoring and recovery of Azure-II (Az-II) using ultrasound assisted dispersive -magnetic nanocomposites-solid-phase microextraction (UA-DMNSPME) based on Zn@Cu-Fe2O4-NCs-CNT sorbent. The full contribution of conventional factors like pH, sorbent amount, eluent volume and sonication time on response were studied and optimized using response surface methodology (RSM) combined with central composite design (CCD). According best figures of merits were achieved at following conditions: pH of 8.0; 5.0 mg sorbent and 5 min sonication which all accumulation Az-II was efficiently eluted by 180 mu L of dimethylformamide which consequently permit achievement recovery of 99.8 +/- 1.1%. Under specified best conditions using 10.0 mL of sample at various conditions lead to detection limit (based on 3S(b)/m), limit of quantification (10S(b)/m) and the enrichment factor of 3.8, 12.6 ng mL(-1) and 75.2, respectively. The while method has linear response over 30-4500 ng mL(-1) of Az-II with acceptable correlation coefficient of R-2 = 0.9996. Inter- and Intra-day precision expressed as relative standard deviation (n = 3) and the preconcentration factor were 1.1-4.0%, 1.4-5.0% and 55.6, respectively. Good recoveries (95.2-103.3%) with low relative standard deviations (1.1-5.0%) strongly support successful applicability of present method for Az-Il preconcentration and determination.
Title: The Effect of Cu-BPDCA-Ty on Antibacterial Activity and The Expression of mecA Gene in Clinical and Standard Strains of Methicillin-Resistant Staphylococcus aureus
Authors: Askarinia, M (Askarinia, Marzieh); Ghaedi, M (Ghaedi, Mehrorang); Manzouri, L (Manzouri, Leila); Khoramrooz, SS (Khoramrooz, Seyed Sajjad); Sharifi, A (Sharifi, Asghar); Ghalamfarsa, G (Ghalamfarsa, Ghasem); Jannesar, R (Jannesar, Ramin); Sadri, F (Sadri, Farzad); Khosravani, SA (Khosravani, Seyed Abdolmajid)
Background: The antibiotic resistance of bacteria has increased in the last decade. The mecA gene plays an important role in the pathogenicity of Methicillin-Resistant Staphylococcus aureus (MRSA) by increasing antibiotics resistance. Recent studies have indicated that nanotechnology, as an antimicrobial agent, has had promising results.
Objectives: The present study was conducted to determine the effect of Cu-BPDCA-Ty on antibacterial activity and mecA gene expression in clinical and standard strains of MRSA.
Methods: The phenotypic tests were used to identify MRSA strain and confirmed with molecular detection of mecA gene. Synthesized Cu-BPDCA-Ty was confirmed with different techniques such as XRD and SEM analysis. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by the micro broth dilution method. Real time PCR was used to investigate gene expression. Pta gene was considered as an endogenous control for normalization. Data were analyzed using one sample t test and paired t test in the SPSS software Version 22.
Results: The findings indicated that the MIC and the MBC of Cu-BPDCA-Ty against the standard and clinical strains of MRSA were 0.5 mg/mL, 0.8 mg/mL, 0.46 +/- 0.08 mg/mL, and 0.7 +/- 0.1 mg/mL, respectively. Analysis of the real-time PCR indicated that all treated groups with Cu-BPDCA-Ty showed a significant decrease in the expression of the mecA gene compared to the control group (P < 0.05).
Conclusions: Cu-BPDCA-Ty had an antibacterial effect on MRSA and induced downregulation of expression of the mecA gene.
Title: Statistical optimization and modeling approach for azo dye decolorization: Combined effects of ultrasound waves and nanomaterial-based adsorbent
Authors: Pooralhossini, J (Pooralhossini, Jaleh); Zanjanchi, MA (Zanjanchi, Mohammad Ali); Ghaedi, M (Ghaedi, Mehrorang); Asfaram, A (Asfaram, Arash); Azqhandi, MHA (Azqhandi, Mohammad Hossein Ahmadi)
This study is devoted to an investigation of the effects of sonication time, adsorbent mass, pH and sunset yellow (SY) and disulfine blue (DB) concentration on the removal of DB and SY from water. Artificial neural network and response surface methodology approaches were used to optimize an analytical model to calculate the DB and SY removal performance of tin oxide nanoparticles loaded on activated carbon. The performance of both models was statistically evaluated in terms of the coefficient of determination (R-2), root mean squared error (RMSE), mean absolute error (MAE) and absolute average deviation (AAD), and graphical plots were also used for comparison of the models. The obtained results show that the artificial neural network model outperforms the classical statistical model in terms of R-2, RMSE, MAE and AAD for both dyes. Various isotherm models were studied for fitting the experimental equilibrium data, and the results confirm the applicability of the Langmuir isotherm for description of the adsorption equilibrium. Various kinetic models were applied to the experimental data and the results reveal that the pseudo-second-order model with better correlation is superior to the other kinetic models. The significant factors were optimized using the desirability function approach combined with central composite design. The obtained optimal point is located in the valid region and the experimental confirmation indicates good agreement between the predicted optimal points and the experimental data.
Title: Podophyllotoxin extraction from Linum usitatissimum plant and its anticancer activity against HT-29, A-549 and MDA-MB-231 cell lines with and without the presence of gold nanoparticles
Authors: Safarpoor, M (Safarpoor, Mohammad); Ghaedi, M (Ghaedi, Mehrorang); Yousefinejad, M (Yousefinejad, Masoume); Javadian, H (Javadian, Hamedreza); Asfaram, A (Asfaram, Arash); Ghasemi, Z (Ghasemi, Zahra); Jaberi, H (Jaberi, Hajar); Rahimi, D (Rahimi, Daruosh)
In recent years, gold nanoparticles (Au-NPs) have been taken into consideration in nanomedicine due to their excellent biocompatibility, chemical stability and promising optical properties. In this research, podophyllotoxin conjugated with gold nanoparticles (Au-NPs-POT) was synthesized and the conjugation of POT with Au-NPs was confirmed using scanning electron microscopy, mass spectrometry and Fourier transform infrared spectroscopy. The anticancer effects of the product on preclinical models of lung, colon and breast cancers were investigated using MTT test. The analyses showed a direct dose-response relationship. It was found that higher concentrations of POT have more positive effects on the inhibition of cancer cell growth. At POT concentrations of 1, 2.5, 5, 7.5, 10, 15 and 20ng ml(-1), approximately 50% of the growth of colorectal, lung and breast cancer cell lines was inhibited, while similar results were obtained in the presence of 1, 2, 3, 4 and 5 g ml(-1) Au-NPs-POT. Au-NPs-POT exhibited the lowest cytotoxicity due to the presence of POT. The anticancer feature of Au-NPs-POT proved the potential to develop better anticancer therapeutics and to open new avenues for treatment of cancers.
Title: Zinc oxide nanorod-loaded activated carbon for ultrasound-assisted adsorption of safranin O: Central composite design and genetic algorithm optimization
Authors: Sharifpour, E (Sharifpour, E.); Ghaedi, M (Ghaedi, M.); Azad, FN (Azad, F. Nasiri); Dashtian, K (Dashtian, K.); Hadadi, H (Hadadi, H.); Purkait, MK (Purkait, M. K.)
This paper focuses on the development of an effective methodology to obtain the optimum ultrasonic-assisted removal of a dye, safranin O (SO), under optimum conditions that maximize the removal percentage, using ZnO nanorod-loaded activated carbon (ZnO-NRs-AC) in aqueous solution. Central composite design coupled with genetic algorithm was used for parameter optimization. The effects of variables such as pH, initial dye concentration, mass of ZnO-NRs-AC and sonication time were studied. The interactive and main effects of these variables were evaluated using analysis of variance. The structural and physicochemical properties of the ZnO-NRs-AC adsorbent were investigated using field emission scanning electron microscopy and X-ray diffraction. Adsorption equilibrium data were fitted well with the Langmuir isotherm and the maximum monolayer capacity was found to be 32.06 mg g(-1). Studies of the adsorption kinetics of the SO dye showed a rapid sorption dynamic with a (pseudo-)second-order kinetic model, suggesting a chemisorption mechanism.
Title: Modified dispersive liquid-phase microextraction based on sequential injection solidified floating organic drop combined with HPLC for the determination of phenobarbital and phenytoin
Authors: Pebdani, AA (Pebdani, Arezou Amiri); Dadfarnia, S (Dadfarnia, Shayessteh); Shabani, AMH (Shabani, Ali Mohammad Haji); Khodadoust, S (Khodadoust, Saeid); Talebianpoor, MS (Talebianpoor, Mohammad Sharif)
A modified dispersive liquid phase microextraction based on sequential injection solidified floating organic drop was developed for simultaneous separation/ preconcentration of trace amounts of phenobarbital and phenytoin. The important factors affecting on the extraction recovery including pH, the volume of extraction solvent, ionic strength, and the number of injections were investigated and optimized by Box-Behnken design and desirability function. Under the optimum experimental conditions, the calibration graph was linear in the concentration range of 1.0-300.0 mu g/L (r(2) = 0.997) for phenobarbital and 2.0-400.0 mu g/L (r(2) = 0.996) for phenytoin. The limit of detection and limit of quantification were 0.35 and 1.2 mu g/L for phenobarbital and 0.65 and 2.2 mu g/L for phenytoin, respectively. The relative standard deviation for six replicate determinations at 10 mu g/L was 3.3 and 4.1% for phenobarbital and phenytoin, respectively. The developed method was successfully applied to the determination of phenobarbital and phenytoin in urine and plasma samples.
Title: Isotherms and kinetic study of ultrasound-assisted adsorption of malachite green and Pb2+ ions from aqueous samples by copper sulfide nanorods loaded on activated carbon: Experimental design optimization
Authors: Sharifpour, E (Sharifpour, Ebrahim); Khafri, HZ (Khafri, Hossein Zare); Ghaedi, M (Ghaedi, Mehrorang); Asfaram, A (Asfaram, Arash); Jannesar, R (Jannesar, Ramin)
Copper sulfide nanorods loaded on activated carbon (CuS-NRs-AC) was synthesized and used for simultaneous ultrasound-assisted adsorption of malachite green (MG) and Pb2+ ions from aqueous solution. Following characterization of CuS-NRs-AC were investigated by SEM, EDX, TEM and XRD, the effects of pH (2.0-10), amount of adsorbent (0.003-0.011 g), MG concentration (5-25 mg L-1), Pb2+ concentration (3-15 mg L-1) and sonication time (1.5-7.5 min) and their interactions on responses were investigated by central composite design (CCD) and response surface methodology. According to desirability function on the Design Expert optimum removal (99.4% +/- 1.0 for MG and 68.3 +/- 1.8 for Pb2+ ions) was obtained at pH 6.0, 0.009 g CuS-NRs-AC, 6.0 min mixing by sonication and 15 and 6 mg L-1 for MG and Pb2+ ions, respectively. High determination coefficient (R-2 > 0.995), Pred-R-2-value (> 0.920) and Adju-R-2-value (> 0.985) all are good indication of best agreement between the experimental and design modelling. The adsorption kinetics follows the pseudo-second order model and adsorption isotherm follows the Langmuir model with maximum adsorption capacity of 145.98 and 47.892 mg g(-1) for MG and Pb2+ ions, respectively. This adsorbent over short contact time is good choice for simultaneous removal of large content of both MG and Pb2+ ions from wastewater sample.
Title: Synthesis, antimicrobial evaluation and docking studies of some novel quinazolinone Schiff base derivatives
Authors: Nasab, RR (Nasab, Rezvan Rezaee); Mansourian, M (Mansourian, Mahboubeh); Hassanzadeh, F (Hassanzadeh, Farshid)
The quinazolin-4(3H)-one structural motif possesses a wide spectrum of biological activities. DNA gyrase play an important role in induction of bacterial death. It has been shown that many quinazolin-4(3H)-one derivatives have antibacterial effects through inhibition of DNA gyrase. Based on this information we decided to synthesize novel quinazolinone Schiff base derivatives in order to evaluate their antibacterial effects. A series of novel quinazolinone Schiff base derivatives were designed and synthesized from benzoic acid. The potential DNA gyrase inhibitory activity of these compounds was investigated using in silico molecular docking simulation. All new synthesized derivatives were screened for their antimicrobial activities against three species of Gram-negative bacteria including Escherichia coli, Pseudomonas aeruginosa, Salmonella entritidis and three species of Gram-positive bacteria comprising of Staphylococcus aurous, Bacillus subtilis, Listeria monocitogenes as well as for antifungal activities against Candida albicans using the conventional micro dilution method. Most of the compounds have shown good antibacterial activities, especially against E. coli at 128 mu g/mL concentration while no remarkable antifungal activities were observed for these compounds. All the synthesized compounds exhibit dock score values between -5.96 and -8.58 kcal/mol. The highest dock score among them was -8.58 kcal/mol for compound 4c.
Title: A study on OPG/RANK/RANKL axis in osteoporotic bile duct-ligated rats and the involvement of nitrergic and opioidergic systems
Authors: Doustimotlagh, AH (Doustimotlagh, Amir Hossein); Dehpour, AR (Dehpour, Ahmad Reza); Etemad-Moghadam, S (Etemad-Moghadam, Shahroo); Alaeddini, M (Alaeddini, Mojgan); Ostadhadi, S (Ostadhadi, Sattar); Golestani, A (Golestani, Abolfazl)
Chronic liver disease (CLD) affects millions of people and its impact on bone loss has become a subject of interest. Nitric oxide and endogenous opioids are suggested to increase during cholestasis/cirrhosis and may impact bone resorption by different mechanisms. The receptor activator of nuclear factor-kappa B (RANK)/RANK-ligand (RANKL)/osteoprotegerin (OPG) signaling pathway regulates bone resorption, but its role in metabolic bone disease subsequent to CLD is unknown. We aimed to investigate the involvement of nitrergic and opioidergic systems in bone loss relative to the RANK/RANKL/OPG pathway, in bile ductligated (BDL) rats. Eighty BDL/sham-operated (SO) rats received injections of 3 mg/kg/day N omega-Nitro-L-arginine methyl ester +/- naltrexone (10 mg/kg/day) or saline for 28 days. Plasma bone turnover markers, OPG, RANK, and RANKL along with mRNA expression levels of the latter three were assessed. Plasma bone turnover markers and OPG level increased, but RANKL decreased in the BDL group compared with their SO controls (both: P <= 0.05). Administration of naltrexone reduced bone turnover markers and OPG level while increased RANKL content in comparison to BDL rats (P <= 0.05). As compared to untreated BDL rats, nitric oxide inhibition showed no effect on bone turnover marker i.e. OPG, RANK, and RANKL levels. BDL significantly increased RANK mRNA, but had no significant effect on RANKL and OPG mRNA expression. The lack of association between plasma levels and quantitative gene expression of RANKL and OPG suggests an indirect function of these markers in BDL rats. Considering that opioid receptor blockage by naltrexone in BDL animals caused a significant decrease in OPG and an increase in RANKL plasma contents, it could be postulated that the opioidergic system may have a regulatory effect on these bone markers.
Title: Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA
Authors: Sadeghpour, H (Sadeghpour, Hossein); Khalvati, B (Khalvati, Bahman); Entezar-Almahdi, E (Entezar-Almahdi, Elaheh); Savadi, N (Savadi, Narjes); Alhashemi, SH (Alhashemi, Samira Hossaini); Raoufi, M (Raoufi, Mohammad) ; Dehshahri, A (Dehshahri, Ali)
The objective of the present study is to conjugate L-thyroxine PEI derivative onto another PEI to compensate the amine content of the whole structure which has been utilized for the ligand conjugation. Since alpha(v)beta(3) integrin receptors are over-expressed on cancer cells and there is binding site for L-thyroxine on these receptors, PEI conjugation by L-thyroxine along with restoring the PEI amine content might be an efficient strategy for targeted delivery using polymeric nanoparticles. The results demonstrated the ability of the PEI conjugate in the formation of nanoparticles with the size of around 210 nm with higher buffering capacity. The conjugated PEI derivative increased the transfection efficiency in the cell lines over-expressing integrin by up to two folds higher than unmodified PEI, whereas in the cell lines lacking the integrin receptors there was no ligand conjugation-associated difference in gene transfer ability. The specificity of transfection demonstrated the delivery of plasmid DNA through integrin receptors. Also, the results of in vivo imaging of the polyplexes revealed that Tc-99m-labeled PEI/plasmid DNA complexes accumulated in kidney and bladder 4 h post injection. Therefore, this PEI derivative could be considered as an efficient targeted delivery system for plasmid DNA.
Title: Synthesis of CuS nanoparticles loaded on activated carbon composite for ultrasound-assisted adsorption removal of dye pollutants: Process optimization using CCD-RSM, equilibrium and kinetic studies
Authors: Dastkhoon, M (Dastkhoon, Mehdi); Ghaedi, M (Ghaedi, Mehrorang); Asfaram, A (Asfaram, Arash); Javadian, H (Javadian, Hamedreza)
In this study, the CuS nanoparticles loaded on activated carbon (CuS-NPs-AC) composite was synthesized and then, characterized by XRD and FE-SEM analyses. The prepared composite was used as a potential adsorbent for the simultaneous ultrasound-assisted removal of Indigo Carmine (1C) and Safranin-O (SO). The CuS-NPs-AC dose (0.01-0.03 g), sonication time (1-5 min), initial SO concentration (5-15 mg L-1) and initial IC concentration (5-15 mg L-1) as expectable effective parameters were studied by central composite design (CCD) under response surface methodology (RSM) to obtain an useful knowledge about the effect of simultaneous interaction between IC and SO on their removal percentage. The optimum SO and IC removal percentages were determined to be 98.24 and 97.15% at pH = 6, 0.03 g of the CuS-NPs-AC, 3 min sonication time, 12 and 10 mg L-1 of IC and SO. The values of coefficient of determination (R-2) for SO and IC were 0.9608 and 0.9796, respectively, indicating the favorable fitness of the experimental data to the second order polynomial regression model. The isotherm data were well correlated with Freundlich model. The maximum monolayer adsorption capacities of 87.5 and 69.90 mg g(-1) at room temperature for IC and SO in the investigated binary system expressed the high efficiency of the novel adsorbent for water cleanup within a short time. The investigation of correlation between time and rate of adsorption revealed that IC and SO adsorption onto the CuS-NPs-AC followed pseudo-second-order and intra-particle diffusion simultaneously.
Title: Preparation and Characterization of Mn0.4Zn0.6Fe2O4 Nanoparticles Supported on Dead Cells of Yarrowia lipolytica as a Novel and Efficient Adsorbent/Biosorbent Composite for the Removal of Azo Food Dyes: Central Composite Design Optimization Study
Authors: Asfaram, A (Asfaram, Arash); Ghaedi, M (Ghaedi, Mehrorang); Dashtian, K (Dashtian, Kheibar); Ghezelbash, GR (Ghezelbash, Gholam Reza)
The removal of hazardous dyes is of great importance to making healthy and drinkable water. Here, a new ferro-magnetic composite based on Mn0.4Zn0.6Fe2O4 nanoparticles (NPs) supported on dead Yarrowia lipolytica ISF7 (D-YL-ISF7) was prepared. Nanoparticle aggregation was inhibited using D-YL-ISF7, which causes the availability of more active sites. The dead D-YL-ISF7-supported Mn0.4Zn0.6Fe2O4 nanoparticles (NPs) were characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM), energy dispersive X-ray (EDX), Brunuaer-Emmett-Teller (BET), and vibrating sample magnetometer (VSM) analysis and used as robust adsorbents/biosorbents to simultaneously remove tartrazine (TA) and ponceau 4R (P4R) azo food dyes in their binary solution. First order derivative spectrophotometry was implemented for the simultaneous analysis of dyes in binary mixtures. Central composite design (CCD) was used to evaluate the influence of pH, sonication time, Mn0.4Zn0.6Fe2O4-NPs-D-YL-ISF7 mass, and initial TA and P4R concentrations on the efficiency for the removal of the studied dyes. At optimum conditions (pH 2.0, sonication time 5 min, Mn0.4Zn0.6Fe2O4-NPs-D-YL-ISF7 mass 0.015 g, TA concentration 12 mg and P4R concentration 16 mg L-1 high removal efficiencies (>99.0%) were obtained for TA and P4R dyes, reasonably well predicted by the model. The CCD allowed the optimization and the scale-up of the process, which presented a good correlation between large and small scales. Adsorption isotherm data fitted well to the Langmuir model. Under ultrasound, the Langmuir adsorption capacity of MMn0.4Zn0.6Fe2O4-NPs-D-YL-ISF7 was obtained to be 90.827 mg g(-1) for TA and 101.461 mg g(-1) for P4R. A pseudo-second-order reaction model was chosen for kinetic study.
Title: Ultrasound-assisted extraction of antimicrobial compounds from Thymus daenensis and Silybum marianum: Antimicrobial activity with and without the presence of natural silver nanoparticles
Authors: Safarpoor, M (Safarpoor, Mohammad); Ghaedi, M (Ghaedi, Mehrorang); Asfaram, A (Asfaram, Arash); Yousefi-Nejad, M (Yousefi-Nejad, Masoumeh); Javadian, H (Javadian, Hamedreza); Khafri, HZ (Khafri, Hossein Zare); Bagherinasab, M (Bagherinasab, Marzieh)
The present study is devoted to prepare a new antibacterial and antifungal agent based on in situ-synthesized silver nanoparticles at room temperature using Rosmarinus officinalis (R. officinalis) leaf extract. The Ag-NPs characterization by UV-visible, SEM, TEM and XRD revealed that the particles sizes were in the range of 10-33 nm. In this study, hydroalcoholic extracts were used with ultrasonic method. Ultrasonication has recently received attention as a novel bioprocessing tool for process intensification in many areas of downstream processing. The antimicrobial activities of T. daenensis and S. marianum extracts with and without the presence of Ag-NPs were investigated at concentrations from 12.5 to 50 mg/mL against Staphylococcus aureus (S. aureus, Gram-positive organism) and Escherichia coli (E. coli, Gram-negative organism), and fungal strains were Aspergillus oryzae (A. oryzae) and Candida albicans (C. albicans). Antimicrobial activity determined using agar disc diffusion method revealed that the activities of Ag-NPs/T. daenensis were superior to Ag-NPs/S. marianum and extracts (T. daenensis and S. marianum). The medicinal plant extract can be used to synthesize the Ag-NPs as an eco-friendly and inexpensive method in large scale. The results showed that the prepared Ag-NPs/extracts as good antibacterial and antifungal agents can be potentially applied against rapidly increasing of antibiotic resistance.
Title: Essential Oil Composition of Aerial Parts of Vitex pseudo-negundo Populations Collected from Southwest of Iran
Authors: Salehpour, Zeinab, Esfandiar Jahantab, Mohammad Reza Morshedloo, Aida Iraji, and Jamshid Mohamadi.
Vitex pseudo-negundo (Hausskn.) is a perennial plant of Lamiaceae family which grows naturally in the vicinity of seasonal rivers of Iran. The present study was aimed to characterize the essential oils composition of five Iranian populations of V. pseudo-negundo. The aerial parts of the plant were collected at flowering stage. GC-FID and GC-MS analyses of the essential oils identified a total of forty-one components in the examined essential oils, accounting for 95.8- 98.5% of the total composition. According to the results, no significant differences was observed among the main compositions of the essential oils. In all investigated populations, the main fraction was represented by monoterpene hydrocarbons with α-pinene (25.3-29.2%) and limonene (7.89-9.85%) as the most abundant components. (E)-β-methyl ionone (12.38-17.37%), (E)-β-caryophyllene (3.7-9.8%) and β-eudesmol (5.0-6.1%) were the other main components of the essential oils.
Title: Nephroprotective Effect of Nasturtium officinale (Watercress) Ethanol Extract and Vitamin E on Vancomycin-Induced Nephrotoxicity in Rats
Authors: Masoumeh Karami, Mostafa Mostafazade, Hossein Sadeghi, Heibatollah Sadeghi, Fouad Mehraban, Esmaeel Panahi Kokhdan, Moslem Sayahi and Seyed Reza Abtahi
Background: Vancomycin (VCM) is an important antibiotic that is active against gram-positive cocci, and its nephrotoxicity remain as a major problem in clinical use.
Objectives: This study was designed to investigate the effect of Nasturtium officinale hydro-alcoholic extract (NOE) and vitamin E aganist VCM-induced nephrotoxicity in adult male wistar rats.
Methods: A total of 36 animals were randomly divided into 6 equal groups (n = 6) including 1, control group; 2, VCM group; 3, VCM + NOE (250 mg/kg) group; 4, VCM + NOE (500 mg/kg) group; 5, VCM + vitamin E (250 mg/kg) group; and 6, VCM + vitamin E (500 mg/kg) group. VCM (200 mg kg-1 i.p.) was given every 12 hours for 7 consecutive days. NOE and vitamin E were orally given to rats 30 minutes prior to i.p. injection of VCM. Acute renal failure was evaluated by measuring serum urea, creatinine levels, and creatinine clearance. Change in body weight and kidney weight (g/100 g body weight) were measured. The right kidney was fixed in formalin for pathological evaluation and the left kidney was homogenized for determination of malondialdehyde (MDA).
Results: VCM significantly increased serum creatinine and urea levels, MDA levels, kidney weight/100 mg weight of body, as well as reduced creatinine clearance. NOE (250, 500 mg kg-1) and vitamin E (500 mg kg-1) pretreatment considerably alleviated all of these changes when compared with VCM treated alone. Histological examination of VCM-treated group showed a marked renal injury with tubular epithelial cell desquamation, swelling, and tubular dilatation. These changes were mitigated with NOE- and vitamin E.
Conclusions: The data indicate that NOE obviously attenuated VCM-induced nephrotoxicity. Elucidation of the exact mechanism(s) of this protection requires more research, however, it might be mediated by antioxidant effects of NOE.
Title: Cu- and S- @SnO2 nanoparticles loaded on activated carbon for efficient ultrasound assisted dispersive mu SPE-spectrophotometric detection of quercetin in Nasturtium officinale extract and fruit juice samples: CCD-RSM design
Authors: Asfaram, A (Asfaram, Arash); Ghaedi, M (Ghaedi, Mehrorang; Javadian, H (Javadian, Hamedreza); Goudarzi, A (Goudarzi, Alireza)
A simple, rapid, and efficient method of dispersive micro solid phase extraction (D-p-SPE) combined with UV-Vis spectrophotometry via ultrasound-assisted (UA) was applied for the determination and preconcentration of quercetin in extract of watercress (Nasturtium officinale), fruit juice and water samples. The sorbent in this method was synthesized by doping copper and sulfide into the tetragonal structure of SnO2-nanoparticles (Cuand S- @SnO2-NPs) and subsequently loading it on activated carbon (AC). The D-mu-SPE parameters with direct effect on the extraction efficiency of the targeted analyte, such as sample pH, volume of eluent, sorbent mass and ultrasound time were optimized using central composite design method. Under optimized conditions, the calibration graph for quercetin was linear in the range of 20-4000 ng mL(-1); the limit of detection and quantitation were 4.35 and 14.97 ng mL(-1), respectively and the enrichment factor was 95.24. Application of this method to analyze spiked extract, fruit juice and water samples resulted in acceptable recovery values ranging from 90.3% to 97.28% with intra-day and inter-day relative standard deviation values lower than 6.0% in all cases. Among the equilibrium isotherms tested, Langmuir was found to be the best fitted model with maximum sorption capacity of 39.37 mg g(-1), suggesting a homogeneous mode of sorption for quercetin.
Title: Anti-angiogenic effects of CD73-specific siRNA-loaded nanoparticles in breast cancer-bearing mice
Authors: Ghalamfarsa, G (Ghalamfarsa, Ghasem); Rastegari, A (Rastegari, Ali; Atyabi, F (Atyabi, Fatemeh); Hassannia, H (Hassannia, Hadi); Hojjat-Farsangi, M (Hojjat-Farsangi, Mohammad); Ghanbari, A (Ghanbari, Amir); Anvari, E (Anvari, Enayat); Mohammadi, J (Mohammadi, Jamshid); Azizi, G (Azizi, Gholamreza); Masjedi, A (Masjedi, Ali); Yousefi, M (Yousefi, Mehdi); Yousefi, B (Yousefi, Bahman); Hadjati, J (Hadjati, Jamshid); Jadidi-Niaragh, F (Jadidi-Niaragh, Farhad)
CD73 facilitates tumor growth by upregulation of the adenosine (immunosuppressive factor) in the tumor microenvironment, however, its precise molecular mechanisms is not precisely understood. Regarding the importance of angiogenesis in tumor development and spreading, we decided to assign the anti-angiogenic effects of CD73 suppression. We used chitosan lactate (ChLa) nanoparticles (NPs) to deliver CD73-specific small interfering RNA (siRNA) into cancer cells. Our results showed that treatment of the 4T1 cells with CD73-specific siRNA-loaded NPs led to potent inhibition of cancer cell proliferation and cell cycle arrest, in vitro. This growth arrest was correlated with downregulation of angiogenesis-related molecules including vascular endothelial growth factor (VEGF)-A, VEGF-R2, interleukin (IL)-6, and transforming growth factor (TGF)-. Moreover, administration of NPs loaded with CD73-siRNA into 4T1 breast cancer-bearing mice led to tumor regression and increased mice survival time accompanied with downregulation of angiogenesis (VEGF-A, VEGF-R2, VE-Cadherin, and CD31) and lymphangiogenesis (VEGF-C and LYVE-1)-related genes in the tumor site. Furthermore, the expression of angiogenesis promoting factors including IL-6, TGF-, signal transducer, and activator of transcription (STAT)3, hypoxia inducible factor (HIF)-1, and cyclooxygenase (COX)2 was decreased after the CD73 suppression in mice. Moreover, analysis of leukocytes derived from the tumor samples, spleen, and regional lymph nodes showed that they had lower capability for secretion of angiogenesis promoting factors after CD73-silencing. These results indicate that suppression of tumor development by downregulation of CD73 is in part related to angiogenesis arrest. These findings imply a promising strategy for inhibiting tumor growth accompanied with suppressing the angiogenesis process.
Title: Development of an eco- friendly approach based on dispersive liquid- liquid microextraction for the quantitative determination of quercetin in Nasturtium officinale, Apium graveolens, Spinacia oleracea, Brassica oleracea var. sabellica, and food samples
Authors: Arabi, M (Arabi, Maryam); Ostovan, A (Ostovan, Abbas); Asfaram, A (Asfaram, Arash); Ghaedi, M (Ghaedi, Mehrorang)
In this work, a fast, sensitive, inexpensive and environment-friendly dispersive liquid-liquid microextraction (DLLME) technique was developed based on solidification of floating organic drops (SFOD) for the preconcentration of quercetin followed by HPLC-UV. The parameters affecting the extraction efficiency (the type of extractant and disperser solvents and their volume, sample pH, concentration of salt, and sonication time) in the DLLME-SFOD-HPLC-UV method were studied and optimized with the aid of experimental designs. The optimum conditions found were as follows: extractant solvent type and amount, 0.25 mL of dodecanol, disperser solvent type and amount, 0.70 mL of acetonitrile, pH 5.0, extraction time of 0.5 min, and no need of adding salt. Under the optimal conditions, a good linearity in the range of 0.8-3500 g L-1 was reached; furthermore, our proposed method had a reasonable reproducibility (RSD% 1.94-5.20), repeatability (RSD% 1.01-2.82), and recovery (96.65-106.76%). In addition, a low LOD (0.149-0.165 g L-1), high enrichment factor, and very little extraction time were attained for different aqueous matrices (Nasturtium officinale, Apium graveolens, Brassica oleracea var. sabellica, Spinacia oleracea and food samples).
Title: Activation of the Glutathione Peroxidase by Metformin in the Bile-duct Ligation-induced Liver Injury: In vivo Combined with Molecular Docking Studies
Authors: Mansourian, Mahboubeh; Sadeghi, Hossein; Doustimotlagh, Amir Hossein
Journal: Current Pharmaceutical Design Volume: 24 Issue: 27 Pages: 3256-3263
Background: Inhibition of hepatic fibrosis is an attainable objective in managing the chronic liver disease. The present study aimed to investigate possible defensive effects of metformin on the activities of antioxidant enzymes, hydroxyproline content, and biochemical factors in bile duct ligation (BDL)-induced cholestatic rats. The interactive behavior of metformin with glutathione peroxidase (GPx) enzyme was also explained by molecular docking and conformation characterization.
Methods: The present study was conducted on 28-adult male Wistar rats classified into four 7-animal groups: sham-control, mere BDL, and BDL+ metformin that received daily metformin as gavage in two doses of 250 and 500 mg/kg bw for 10 days. Biochemical analysis, hydroxyproline content, and antioxidant enzymes activity were also determined.
Results: The hydroxyproline content significantly increased, but the GPx enzyme activity significantly decreased in the hepatic tissue following BDL, indicating that an oxidative stress-related model in rats was successfully constituted. Administration of metformin at two doses attenuated hydroxyproline content in the cholestatic liver and ameliorated the depletion of GPx enzyme activities compared to the non-treated BDL group (P-value <= 0.05). Molecular docking study provides the evidence for metformin ability to regulate enzymatic activity of GPx.
Conclusion: The research data indicated that due to novel hepatoprotective effects of metformin in an animal model with BDL-induced liver injury, it was a potential beneficial therapeutic agent for treating the cholestatic liver disease. The main mechanism might contribute to antioxidant actions, particularly via GPx enzyme.
Title: A New Spacer Device with Best Quality for Asthma and COPD (RESPIPARD)
Authors: Ghahramanifar, Mehdi; Marioryad, Hossein; Moghadam, Ali Ghadimi; et al
Journal: International Journal of Advanced Biotechnology and Research Volume: 9 Issue: 1 Pages: 322-326 Published: 2018
Title: Study of clinical and Paraclinical findings in children with Kawasaki disease admitted to Imam Sajjad Hospital in Yasuj from 2012 to 2016
Authors: Ghahramanifar, Mehdi; Taheripour, Mahbobeh; Ghatee, Mohammad Amin; et al
Journal: International Journal of Advanced Biotechnology and Research Volume: 9 Issue: 1 Pages: 327-332 Published: 2018
Title: A molecularly imprinted polymer coupled with high-performance liquid chromatography-UV for the determination of albendazole in plasma and urine samples: CCD-RSM design
Authors: Abidi, H (Abidi, Hassan); Ghaedi, M (Ghaedi, Mehrorang); Rafiei, A (Rafiei, Abdollah); Jelowdar, A (Jelowdar, Ali); Arabi, M (Arabi, Maryam); Ostovan, A (Ostovan, Abbas); Asfaram, A (Asfaram, Arash).
Journal: New Journal of Chemistry Volume: 42 Issue: 19 Pages: 15937-15945 Published: OCT 7 2018
Title: Simple and selective detection of quercetin in extracts of plants and food samples by dispersive-micro-solid phase extraction based on core-shell magnetic molecularly imprinted polymers
Authors: Asfaram, Arash; Arabi, Maryam; Ostovan, Abbas; et al
Journal: New Journal of Chemistry Volume: 42 Issue: 19 Pages: 16144-16153 Published: OCT 7 2018
Title: Latitude, Vitamin D, Melatonin, and Gut Microbiota Act in Concert to Initiate Multiple Sclerosis: A New Mechanistic Pathway
Authors: Ghareghani, Majid; Reiter, Russel J.; Zibara, Kazem; et al
Journal: Frontiers In Immunology Volume: 9 Article Number: 2484 Published: OCT 30 2018
Title: Magnetic solid lipid nanoparticles co-loaded with albendazole as an anti-parasitic drug:reparation, characterization, and in vitro drug release
Authors: Abidi, H (Abidi, Hassan); Ghaedi, M (Ghaedi, Mehrorang); Rafiei, A (Rafiei, Abdollah); Jelowdar, A (Jelowdar, Ali); Salimi, A (Salimi, Anayatollah); Asfaram, A (Asfaram, Arash); Ostovan, A (Ostovan, Abbas).
Journal: Journal of Molecular Liquids Volume: 268 Pages: 11-18 Published: OCT 15 2018
Title: Visible-light-driven photocatalytic degradation of fenpyroximate in rotating packed bed reactor using Fe3O4@PbS@Ni2P magnetic nanocomposite photocatalyst: Response surface modelling and optimization
Authors: Mosleh, Soleiman, Mahmood Reza Rahimi, Mehrorang Ghaedi, Arash Asfaram, Hamedreza Javadian, Fardin Sadeghfar, and Ramin Jannesar.
Journal: Applied Organometallic Chemistry Volume: 32 Issue: 10 Article Number: e4513 Published: OCT 2018
Title: Genetic variants in long noncoding RNA H19 and MEG3 confer risk of type 2 diabetes in an Iranian population
Authors: Ghaedi, Hamid; Zare, Ali; Omrani, Mir Davood; et al
Journal: Gene Volume: 675 Pages: 265-271 Published: OCT 30 2018
Title: Light-Emitting Diode (LED) Therapy Attenuates Neurotoxicity of Methanol-Induced Memory Impairment and Apoptosis in the Hippocampus
Authors: Ghanbari, Amir; Zibara, Kazem; Salari, Sepideh; et al
Journal: CNS & Neurological Disorders-Drug Targets Volume: 17 Issue: 7 Pages: 528-538 Published: 2018
Title: Identification of Candida Species Isolated from Vulvovaginal Candidiasis Patients by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Yasuj Southwestern Iran
Authors: Gharaghani, Maral; Ahmadi, Bahram; Sisakht, Marzie Taheripour; et al.
Journal: Jundishapur Journal of Microbiology Volume: 11 Issue: 8 Article Number: e65359 Published: AUG 2018
Title: Methicillin-Resistant Staphylococcus epidermidis in Iran: A Systematic Review and Meta-Analysis
Authors: Razavi, Shabnam; Dadashi, Masoud; Pormohammad, Ali; et al
Journal: Archives of Clinical Infectious Diseases Volume: 13 Issue: 4 Article Number: e58410 Published: AUG 2018
Title: Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line
Authors: Kokhdan, Esmaeel Panahi; Sadeghi, Hossein; Ghafoori, Hossin; et al
Journal: Research in Pharmaceutical Sciences Volume: 13 Issue: 5 Pages: 404-412 Published: SEP-OCT 2018
Title: The use of starch-modified magnetic Fe-0 nanoparticles for naphthalene adsorption from water samples: Adsorption isotherm, kinetic and thermodynamic studies
Authors: Malekzadeh, Maryam; Nejaei, Arezoo; Baneshi, Mohammad Mehdi; et al
Journal: Applied Organometallic Chemistry Volume: 32 Issue: 8 Article Number: e4434 Published: AUG 2018
Title: Determination of gyrA and parC mutations and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection in Iran
Authors: Mirzaii, Mehdi; Jamshidi, Sanaz; Zamanzadeh, Maryam; et al
Journal: Journal of Global Antimicrobial Resistance Volume: 13 Pages: 197-200 Published: JUN 2018
Title: A rapid and efficient sonophotocatalytic process for degradation of pollutants: Statistical modeling and kinetics study
Authors: Mosleh, Soleiman, Mahmood Reza Rahimi, Mehrorang Ghaedi, Arash Asfaram, Ramin Jannesar, and Fardin Sadeghfar
Journal: Journal of Molecular Liquids Volume: 261 Pages: 291-302 Published: JUL 1 2018
Title: Synthesis of CuS and ZnO/Zn(OH)2 nanoparticles and their evaluation for in vitro antibacterial and antifungal activities
Authors: Khafri, Hossein Zare; Ghaedi, Mehrorang; Asfaram, Arash; et al
Journal: Applied Organometallic Chemistry Volume: 32 Issue: 7 Article Number: e4398 Published: JUL 2018
Title: Nanopowder synthesis of novel Sn(II)-imprinted poly(dimethyl vinylphosphonate) by ultrasound-assisted technique: Adsorption and pre-concentration of Sn(II) from aqueous media and real samples
Authors: Kisomi, Amir Shafiee, Afshin Rajabi Khorrami, Taher Alizadeh, Majid Farsadrooh, Hamedreza Javadian, Arash Asfaram, Shabnam Najafi Asli Pashaki, and Parisa Rafiei.
Journal: Ultrasonics Sonochemistry Volume: 44 Pages: 129-136 Published: JUN 2018
Title: Synthesis of Fe3O4@CuS@Ni2P-CNTs magnetic nanocomposite for sonochemical-assisted sorption and pre-concentration of trace Allura Red from aqueous samples prior to HPLC-UV detection: CCD-RSM design
Authors: Asfaram, Arash; Ghaedi, Mehrorang; Abidi, Hassan; et al
Journal: Ultrasonics Sonochemistry Volume: 44 Pages: 240-250 Published: JUN 2018
Title: Summary of studies on thyme in Iran: an integrated analysis study
Authors: Momeninejad, Mohsen; Shirazi, Hamidreza Ghaffarian; Salehi, Amin; et al
Journal: World Family Medicine Volume: 16 Issue: 4 Pages: 113-117 Published: APR 2018
Title: Ultrasound wave assisted adsorption of congo red using gold-magnetic nanocomposite loaded on activated carbon: Optimization of process parameters
Authors: Dil, Ebrahim Alipanahpour; Ghaedi, Mehrorang; Asfaram, Arash; et al
Journal: Ultrasonics Sonochemistry Volume: 46 Pages: 99-105 Published: SEP 2018
Title: Spasticity Treatment Ameliorates the Efficacy of Melatonin Therapy in Experimental Autoimmune Encephalomyelitis (EAE) Mouse Model of Multiple Sclerosis
Authors: Ghareghani, Majid; Zibara, Kazem; Sadeghi, Hossein; et al
Journal: Cellular and Molecular Neurobiology Volume: 38 Issue: 5 Pages: 1145-1151 Published: JUL 2018
Title: Adenosine and adenosine receptors in the immunopathogenesis and treatment of cancer
Authors: Kazemi, Mohammad H.; Mohseni, Sahar Raoofi; Hojjat-Farsangi, Mohammad; et al
Journal: Journal Of Cellular Physiology Volume: 233 Issue: 3 Pages: 2032-2057 Published: MAR 2018