Title: Application of an optimized dispersive nanomaterial ultrasound-assisted microextraction method for preconcentration of carbofuran and propoxur and their determination by high-performance liquid chromatography with UV detection
Authors: Khodadoust, S (Khodadoust, Saeid); Talebianpoor, MS (Talebianpoor, Mohammad Sharif); Ghaedi, M (Ghaedi, Mehrorang)
An extractionmethod based on dispersive nanomaterial ultrasound-assisted microextraction was used for the preconcentration of carbofuran and propoxur insecticides in water samples prior to high-performance liquid chromatography with UV detection. ZnS: Ni nanoparticles were synthesized based on the reaction of the mixture of zinc acetate and nickel acetate with thioacetamide in aqueous media and then loaded on activated carbon (ZnS: Ni-AC). Different methods were used for recognizing the properties of ZnS: Ni-AC and then this nanomaterial was used for extraction of carbamate insecticide as new adsorbent. The influence of variables on the extraction method (such as amount of adsorbent (mg: NiZnS-AC), pH and ionic strength of sample solution, vortex and ultrasonic time (min), ultrasound temperature and desorption volume (mL) was investigated by a screening 2(7-4) Plackett-Burman design. Then the significant variables were optimized by using a central composite design combined with a desirability function. At optimum conditions, this method had linear response > 0.0060-10 mu g/mL with detection limit 0.0015 mu g/mL and relative standard deviations < 5.0% (n = 3).
Title: Anti-inflammatory Activity of Stachys Pilifera Benth
Authors: Sadeghi, H (Sadeghi, Heibatollah); Zarezade, V (Zarezade, Vahid); Sadeghi, H (Sadeghi, Hossein); Toori, MA (Toori, Mehdi Akbartabar); Barmak, MJ (Barmak, Mehrzad Jafari); Azizi, A (Azizi, Arsalan); Ghavamizadeh, M (Ghavamizadeh, Mehdi); Mostafazadeh, M (Mostafazadeh, Mostafa)
Background: Stachys piliferaBenth has long been used to treat infectious diseases as well as respiratory and rheumatoid disorders in Iranian folk medicine. Antioxidants, antitumor, and antimicrobial properties of the plant have been reported.
Objectives: This experimental study was designed to evaluate systemic and topical anti-inflammatory effects of the hydro-alcoholic extract from aerial parts of Stachys pilifera (HESP).
Materials and Methods: Anti-inflammatory effects of HESP was studied in four well-known animal models of inflammation, including carrageenan-or formalin-induced paw edema in rat (thirteen groups, 6 rats per each group), and 12-O-tetradecanoylphorbol-13-acetate (TPA)- or xylene-induced ear edema in mouse (ten groups, 6 mice per each group). The rats received HESP (50-400 mg/kg) orally 45 minutes before the subplantar injection of carrageenan or formalin. In TPA or xylene tests, HESP (1, 2.5, and 5 mg/ear) was applied topically simultaneous with these phlogistic agents on the ear mice. Finally, pathological examination of the inflamed tissues (paw and ear) was carried out.
Results: Acute toxicity study of the extract showed that no rats were killed at 5000 mg/kg (LD50 > 5000 mg/kg). The extract (100 and 200 mg/kg) significantly suppressed carrageenan-induced paw edema 1, 2, 3, and 4 hours after carrageenan challenge in comparison with the control group (P < 0.001). The HESP (100 and 200 mg/kg) also produced a considerable antiedematogenic effect in the formalin test over a period of 24 hours (P < 0.01). Furthermore, topical administration of the HESP (1, 2.5, and 5 mg/ear) inhibited TPA-and xyleneinduced ear edema in comparison with the control group (P < 0.001). The pathological analysis of the paws and ears revealed that HESP was capable of reducing tissue destruction, cellular infiltration, and subcutaneous edema induced by the indicated phlogistic agents.
Conclusions: The present data confirmed systemic and topical anti-inflammatory effects of Stachys pilifera which is comparable to indomethacin.
Title: Acceleration of methylene blue adsorption onto activated carbon prepared from dross licorice by ultrasonic: Equilibrium, kinetic and thermodynamic studies
Authors: Ghaedi, M (Ghaedi, M.); Ghazanfarkhani, MD (Ghazanfarkhani, M. Danaei); Khodadoust, S (Khodadoust, S.); Sohrabi, N (Sohrabi, N.); Oftade, M (Oftade, M.)
Adsorption of methylene blue (MB) onto activated carbon (AC) prepared from dross licorice as adsorbent was studied following full AC characterization by FT-IR spectroscopy, scanning electron microscopy and BET (Brunauer, Emmett, and Teller). The effects of variables were studied and optimized by a central composite design (CCD) combined with response surface methodology (RSM) and desirability function (DF). The good agreement between experimental and predicted data at optimum values of all variables was explored. The optimum condition set as follow: 20 mg L-1 of MB concentration, pH 7 for sample solution, 0.20 g of AC and 16 min sonication as mass transfer process. The small amount of proposed adsorbent (0.2 g) was applicable for removal of MB (RE > 95%) at 16 min with high adsorption capacity (82.9 mg g-1).
Title: Application of optimized dispersive liquid-liquid microextraction for determination of melatonin by HPLC-UV in plasma samples
Authors: Talebianpoor, MS (Talebianpoor, M. S.); Khodadoust, S (Khodadoust, S.); Rozbehi, A (Rozbehi, A.); Toori, MA (Toori, M. Akbartabar); Zoladl, M (Zoladl, M.); Ghaedi, M (Ghaedi, M.); Mohammadi, R (Mohammadi, R.); Hosseinzadeh, AS (Hosseinzadeh, A. S.)
Melatonin (N-acetyl-3-(2-aminoethyl)-5-methoxyindole) is biologically active as a neurohormone and antioxidant agent. The optimized dispersive liquid-liquid microextraction (DLLME) followed by high-performance liquid chromatography-ultra violet detection (HPLC-UV) was used for the analysis of melatonin in human plasma. Influence variables such as volume of extracting (carbon tetrachloride: CCl4) and dispersing solvents (acetonitrile: ACN), pH and ionic strength, extraction time and centrifugation time were screened in a 2(6-2) fractional factorial design (FFD) and then the significant variables were optimized by using a central composite design (CCD). At optimum conditions values of variables set as pH 6.0, 1.5 mL ACN, 140 mu L CCl4, 1.0 min extraction time and 3.0 min centrifugation at 4500 rpm. At optimum conditions method has linear response over 2.0-500.0 ng mL(-1) with detection limit of 0.5 ng mL(-1) with relative standard deviations (RSDs) less than 5.0%. The values of intra-day and inter-day RSD were 4.3% and 8.5%, respectively. The method was applied successfully for the analysis of melatonin in plasma sample. (C) 2014 Elsevier B.V. All rights reserved.
Title: Testicular morphology of male rats exposed to Phaleria macrocarpa (Mahkota dewa) aqueous extract
Authors: Parhizkar, S (Parhizkar, Saadat); Zulkifli, SB (Zulkifli, Suriani Binti); Dollah, MA (Dollah, Mohammad Aziz)
Objective(s): This study was designed to investigate the effect of Phaleria macrocarpa aqueous extract (PM) on spermatogenesis by observing the histological changes of testes in adult male rats.
Materials and Methods: PM was prepared by boiling the dried slices of P. macrocarpa fruits followed by filtering centrifugation and freeze-drying to obtain the powder form. Eighteen Sprague Dawley adult male rats were divided into three groups (six in each group), designated as treatment (240 mg/kg PM), negative control (distilled water) and positive control (4mg/kg testosterone) and administered via intragastric gavage for seven weeks. In the sixth week of supplementation period, each male rat was introduced to five female rats. Afterward, all rats were sacrificed and the testes were removed for histological studies.
Results: PM significantly increased the number of cell and the thickness of seminiferous tubules of male rats (P<0.05). However, there was no significant effect on the volume and size of testes. The mean of spermatogonia cells numbers of PM groups differed significantly from the negative and positive groups (P<0.05).
Conclusion: PM showed potential value as an attractive alternative for improving sexual strength by increasing the number of spermatogonia cell and the thickness of the seminiferous tubules. Perhaps, PM could be suggested to be one of the herbal remedies that can improve men fertility. The results may have some clinical implication in the management of infertility.
Title: Free radical scavenging activity of homo and copolymer of aniline and para-phenylenediamine prepared in ZnCl2 medium
Authors:Parsa, A (Parsa, Ali); Sadeghi, M (Sadeghi, Maryam); Maleki, M (Maleki, Mostafa); Parhizkar, S (Parhizkar, Saadat); Ab Ghani, S (Ab Ghani, Sulaiman)
This study reports on the ability of homo and copolymer of aniline and para -phenylenediamine (pPDA) to act as antioxidants to free radical 1,1-dipheny1-2-picrylhydrazyl (DPPH). Both the homopolymer and copolymer of aniline and pPDA at different molar ratios are synthesized on composite graphite electrode in phosphoric acid containing 0.5 M zinc chloride. The antioxidant activity has been monitored using UV-visible spectroscopy. The spectra of reaction mixtures in methanol have indicated that the elimination of the DPPH radical is increased with increasing of mole fraction of pPDA monomer. This is also confirmed by electrochemical impedance spectroscopy which demonstrated that as more pPDA are available in the mole fractions, the scavenging of DPPH radicals becomes more efficient. Charge transfer resistance (R-ct) value of polyaniline (PAni) film is 2 x 10(4) Omega, which is much larger than that of poly para-phenylenediamine (PpPDA).
Title: Identification and determination of the fatty acid composition of Quercus brantii growing in southwestern Iran by GC-MS
Authors: Khodadoust, S (Khodadoust, S.); Mohammadzadeh, A (Mohammadzadeh, A.); Mohammadi, J (Mohammadi, J.); Irajie, C (Irajie, C.); Ramezani, M (Ramezani, M.)
This article reports the fatty acid composition of the oil extracts from Quercus brantii fruits growing in Kohgiloye va Boyer Ahmad province in southwestern Iran. The oil from Q. brantii fruits was extracted with hexane in Soxhlet apparatus and subsequently identified and determined by using gas chromatography-mass spectroscopy. The results revealed that the major fatty acids were oleic acid (52.99-66.14%), linoleic acid (10.80-11.11%), palmitic acid (8.08-10.06%), stearic acid (0.74-1.57%), alpha-linolenic acid (0.19-0.35%), erucic acid (0.12-0.15%) and arachidic acid (0.12-0.13%). The total proportion of unsaturated and saturated oil was 64.60-77.27% and 9.17-11.75%, respectively. Results indicate that the fruits of Q. brantii contained 0.19-0.35% omega-3, 10.92-14.77% omega-6 and 53.14-66.26% omega-9. Therefore, Q. brantii can be introduced as rich sources of fatty acid in food dietary and medical health.
Title: Preconcentration of Sn (II) using the methylene blue on the activated carbon and its determination by spectrophotometry method
Authors: Khodadoust, S (Khodadoust, Saeid); Kouri, NC (Kouri, Narges Cham)
A simple and accurate spectrophotometric method for determination of trace amounts of Sn (II) ion in soil sample was developed by using the methylene blue (MB) in the presence of activated carbon (AC) as the adsorbent Solid Phase Extraction (SPE) of Sn (II) and then determined by UV-Vis. The Beer's law is obeyed over the concentration range of 1-80 ng mL(-1) of Sn (II) with the detection limits of 0.34 ng mL(-1). The influence of type and volume of eluent, concentration of MB, pH, and amount of AC on sensitivity of spectrophotometric method were optimized. The method has been successfully applied for Sn (II) ion determination in soil sample.
Title: In vivo anti-inflammatory properties of aerial parts of Nasturtium officinale
Authors: Sadeghi, H (Sadeghi, Heibatollah); Mostafazadeh, M (Mostafazadeh, Mostafa); Sadeghi, H (Sadeghi, Hossein); Naderian, M (Naderian, Moslem); Barmak, MJ (Barmak, Mehrzad Jafari); Talebianpoor, MS (Talebianpoor, Mohammad Sharif); Mehraban, F (Mehraban, Fouad)
Context: Nasturtium officinale R. Br. (watercress) has long been used in Iranian folk medicine to treat hypertension, hyperglycemia, and renal colic. Moreover, anticancer, antioxidant, and hepatoprotective properties of N. officinale have been reported.
Objective: In this study, anti-inflammatory activity of the hydro-alcoholic extract from aerial parts of N. officinale was investigated.
Materials and methods: Oral administration of the hydro-alcoholic extract of N. officinale (250, 500 and 750 mg kg(-1)) was investigated on two well-characterized animal models of inflammation, including carrageenan-or formalin-induced paw edema in rats. Then, the topical anti-inflammatory effect of N. officinale (2 and 5 mg/ear) was studied on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema. Finally, biopsy of the paw or ear was performed for pathological evaluation.
Results: Acute toxicity tests of N. officinale in rats established an oral LD50 of 45 g kg(-1). The extract of watercress (250, 500 and 750 mg kg(-1)) significantly inhibited carrageenan-induced paw edema 1, 2, 3 and 4 h after carrageenan challenge (p<0.001). The extract (500 mg kg(-1)) also showed considerable activity against formalin-evoked paw edema over a period of 24 h (p<0.001). Furthermore, topical application of N. officinale (5 mg/ear) reduced TPA-induced ear edema (p<0.05). Histopathologically, the extract decreased swelling and the tissue damage induced by carrageenan or TPA.
Discussion and conclusion: Our findings indicate potent anti-inflammatory activity of N. officinale in systemic and topical application and propose its potential as an anti-inflammatory agent for treatment of inflammatory conditions.