2019

2019


NO. (1)
Title: Redox imbalance and IL-17 responses in memory CD4(+) T cells from patients with psoriasis

Authors: Esmaeili, B (Esmaeili, Behnaz); Mansouri, P (Mansouri, Parvin); Doustimotlagh, AH (Doustimotlagh, Amir Hossein); Izad, M (Izad, Maryam)

Abstract:
All stages of the inflammatory process involved in T cell-mediated chronic skin disorders like psoriasis are affected by redox imbalance. On the other hand, Th17 cells have a critical role in the pathogenesis of psoriasis. In this study, we evaluated redox status in memory CD4 + T cells and plasma of patients with psoriasis and its correlation with IL-17 response. To this end, memory T cells were isolated from 10 patients with psoriasis and 10 controls. Intracellular Glutathione (GSH), reactive oxygen species (ROS) and superoxide as well as IL-17 were measured using flow cytometry. Plasma total anti-oxidant capacity (TAC) was quantified by ferric reducing ability of plasma (FRAP) assay. The expression of catalase (CAT), superoxide dismutase 1(SOD1), superoxide dismutase 2 (SOD2), nuclear factor, erythroid 2 like 2 (NFE2L2) and cytochrome b-245 beta chain (CYBB) genes were analysed using real-time PCR. Our results showed an increased intracellular ROS production in memory CD4 + T cells of patients compared to controls, (P = 0.04). Furthermore, a significant decrease in expression of catalase gene was found in patients, (P = 0.02). However, no significant differences were observed for intracellular GSH, IL-17 and TAC levels between patients and controls. Also, no correlation was seen between the intracellular IL-17 level and intracellular ROS, GSH and catalase gene expression levels. Collectively, we found an increased ROS production in stimulated memory T cells of patients that could be due to reduced expression of catalase gene. However, it seems that these redox abnormalities have no relationship with IL-17 response in memory T cells.

 

SCANDINAVIAN JOURNAL OF IMMUNOLOGY

Volume: 89

Issue: 1

Article Number: UNSP e12730

DOI: 10.1111/sji.12730

Published: JAN 2019

Impact Factor (2017): 2.314

Quartile in Category: Q3

 


 

NO. (2)
Title: Simultaneous Determination of Two Alcohols and One Anionic Surfactant in Commercial Liquid Cleaners Using Partial Least Square Regression by Fourier Transform Infrared Spectrometry

 

Authors: Mansourian, M (Mansourian, Mahboubeh); Ahmadi, SH (Ahmadi, Seyyed Hamid); Nazifi, M (Nazifi, Maryam); Kargosha, K (Kargosha, Kazem)

 

Abstract:
A simple, fast, efficient, and reagent free procedure without any chemical pretreatment has developed for simultaneous analysis of sodium lauryl ether sulfate (SLES), ethanol and isopropanol in some commercial cleaners. This determination was carried out using attenuated total reflectance Fourier transform infrared spectrometry (ATR-FTIR) and multivariate analysis. Partial leastsquares (PLS) statistical method for quantitative analysis was used to determine the spectra of three components in a mixture of liquid household detergents. The statistical parameters such as Root Mean Square Error of Calibration (RMSEC), Root Mean Square Error of Cross Validation (RMSECV), Standard Errors of Prediction (SEP) and square correlation coefficient (R-2) were obtained 0.07, 0.18, 0.16 (v/v%), 0.15, 0.32, 0.69 (v/v%), 0.22, 0.34, 0.57 (v/v%) and 0.9995, 0.9996 and 0.9998 for SLES, isopropanol and ethanol respectively. Good agreements were achieved between our results and the reference and standard values.

 

TENSIDE SURFACTANTS DETERGENTS

Volume: 56

Issue: 1

Pages: 25-34

DOI: 10.3139/113.110602

Published: JAN 2019

Document Type: Article

Impact Factor (2017): 0.819

Quartile in Category: Q4

 


NO. (3)
Title: Cotoneaster Manna Effects on Animal Model of Aggression and Depression like Behaviors in the Environment

 

Authors: Kharamin, S (Kharamin, Shirali); Mansoorkhani, HS (Mansoorkhani, Heibatollah Sadeghi); Kharamin, R (Kharamin, Roggayeh); Babamir, R (Babamir, Rezvan); Nazir, HMA (Nazir, Hashemi Mohammad Abad)

 

Abstract:
Objective:
complementary medicine (CAM), especially herbal medicine, is widely used in worldwide. Although use of herbal medicine among patients with mental disorders seems common, limited scientific data exist about the efficacy, side effects and safety of these herbs. In other hands, there are also concerns about the possible negative psychiatric effects. Cotoneaster manna, as an herb, has been used pervasively in traditional and modern medicine for treatment of physical disorders as therapeutic and protective medicine. This study was established to assess domestic common believes and concerns (especially psychiatric problem side effects) about this herb and also assess its effects on animal model of aggression and depression Methods and materials: in this experiment 24 male Albumin Wistar rats were divided to three groups, control, treatments (500 and 1000 mg/ kg) and Bilineaster herbal oral drop (Cotoneaster manna drop) was gastrogavaged for five days. Resident intruder paradigm, chronic mild stress, and the forced swim test were administrated and data were calculated according to protocols. Results: ANOVA revealed a significant difference between three group in both of swimming (p=.002) and mobility (p=.004) with higher scores for treatment groups. There was no significant difference between three groups in offensive aggressive behavior and social exploration behaviors in the environment (p>.05). Conclusion: The result showed that Cotoneaster manna not only increased anti-depressive like behaviors, but also does not induce any aggressive behavior in the environment.

 

EKOLOJI

Volume: 28

Issue: 107

Pages: 175-181

Article Number: UNSP e107006

Published:2019

Impact Factor (2016): 0.516

Quartile in Category: Q4



NO. (4)

Title: Effective determination of trace residues of glibenclamide in urine samples using dispersive micro solid-phase extraction and its final detection by chromatographic analysis

 

Authors: Eilami, O (Eilami, Owrang); Asfaram, A (Asfaram, Arash); Ghaedi, M (Ghaedi, Mehrorang); Goudarzi, A (Goudarzi, Alireza)

 

Abstract:
An analytical procedure for rapid and effective dispersive micro-solid-phase extraction (DMSPE) of glibenclamide (GB) from urine samples was set up by using sulfur doped tin oxide nanoparticles (S@ SnO2-NPs) loaded on activated carbon (AC), as the sorbent, and clean-up materials, followed by detection. The main experimental parameters affecting the extraction efficiency for GB were assessed and optimized. The maximum recovery of GB predicted by optimization plots was 98.86%, obtained with 15 mL of water, 250 mL of methanol (as an eluent), pH 8.0, 25 mg of S@ SnO2-NPs-AC, and an extraction time of 2.5 min. Our results indicated that under these optimized extraction conditions, determination coefficients (R-2) of the calibration curves in the range of 0.60-5500 mg L-1 were > 0.991, detection limits (signal-to-noise ratio = 3) were in the range of 0.09-0.16 mg L-1, the average recoveries of spiked GB in urine samples were 95.73-105.50%, and assessed relative standard deviations (RSDs, %) were between 1.15 and 6.84%. The results also demonstrated that the presented method could be suitable for the determination of trace residues of GB in urine samples.

 

ANALYTICAL METHODS

Volume: 11

Issue: 5

Pages: 627-634

DOI: 10.1039/c8ay01965g

Published: FEB 7 2019

Impact Factor (2017): 2.073

Quartile in Category: Q2

 


NO. (5)

Title: Melatonin Therapy Modulates Cerebral Metabolism and Enhances Remyelination by Increasing PDK4 in a Mouse Model of Multiple Sclerosis

 

Authors: Ghareghani, M (Ghareghani, Majid); Scavo, L (Scavo, Linda); Jand, Y (Jand, Yahya); Farhadi, N (Farhadi, Naser); Sadeghi, H (Sadeghi, Hossein); Ghanbari, A (Ghanbari, Amir); Mondello, S (Mondello, Stefania); Arnoult, D (Arnoult, Damien); Gharaghani, S (Gharaghani, Sajjad); Zibara, K (Zibara, Kazem)

 

Abstract:
Metabolic disturbances have been implicated in demyelinating diseases including multiple sclerosis (MS). Melatonin, a naturally occurring hormone, has emerged as a potent neuroprotective candidate to reduce myelin loss and improve MS outcomes. In this study, we evaluated the effect of melatonin, at both physiological and pharmacological doses, on oligodendrocytes metabolism in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS. Results showed that melatonin decreased neurological disability scores and enhanced remyelination, significantly increasing myelin protein levels including MBP, MOG, and MOBP. In addition, melatonin attenuated inflammation by reducing pro-inflammatory cytokines (IL-1 beta and TNF-alpha) and increasing anti-inflammatory cytokines (IL-4 and IL-10). Moreover, melatonin significantly increased brain concentrations of lactate, N-acetylaspartate (NAA), and 3-hydroxy-3-methylglutaryl-coenzyme-A reductase (HMGCR). Pyruvate dehydrogenase kinase-4 (PDK-4) mRNA and protein expression levels were also increased in melatonin-treated, compared to untreated EAE mice. However, melatonin significantly inhibited active and total pyruvate dehydrogenase complex (PDC), an enzyme under the control of PDK4. In summary, although PDC activity was reduced by melatonin, it caused a reduction in inflammatory mediators while stimulating oligodendrogenesis, suggesting that oligodendrocytes are forced to use an alternative pathway to synthesize fatty acids for remyelination. We propose that combining melatonin and PDK inhibitors may provide greater benefits for MS patients than the use of melatonin therapy alone.

 

FRONTIERS IN PHARMACOLOGY

Volume: 10

Article Number: 147

DOI: 10.3389/fphar.2019.00147

Published: FEB 28 2019

Impact Factor (2017): 3.831

Quartile in Category: Q1

 


NO. (6)
Title: Effects of the hydrophilic extract of Juniperus excelsa on renal function in male Wistar rats

 

Authors: Mehrabi, S (Mehrabi, Sadrollah); Checknezhad, H (Checknezhad, Hamideh); Mehrabi, A (Mehrabi, Amir); Vaziri, A (Vaziri, Afshin)

 

Abstract:
Introduction: Ores plant (Juniperus excelsa) has been used for a long-time in the treatment of kidney disease.

Objectives: The aim of this study was to investigate the effects of J. excelsa extract on renal function in male Wistar rats.

Materials and Methods: In this study, 32 male Wistar rats were randomly assigned into four groups of eight rats. Distilled water was used for the healthy control group and the other three groups received doses of 10%, 25% and 50% of the extract for one month. Prior to the intervention and on the 15th and 30th days after intervention, 24-hour urine was collected for measurement of protein, creatinine, and urine volume. On the 30th day, the rats were anesthetized with ether and in addition to the urinary samples, serum samples were taken directly from their heart to check for creatinine, urea, sodium, and potassium. Additionally, both kidneys were removed and examined for histological changes.

Results: There was a significant difference between the groups before and after intervention regarding creatinine clearance (P = 0.008). The mean serum urea on the 15th and 30th days of study was respectively 93 +/- 37.33 and 86.47 +/- 71.07 mg/dL (P = 0.001). In pathology examination, minimal infiltration of inflammatory cells in the interstitium and mild decrease in thickness of renal tubules was observed in 50% dose of the extract.

Conclusion: This study showed that the greatest impact of J. excelsa on the renal function of the male Wistar rats was in doses of 50% of the extracts.

 

JOURNAL OF RENAL INJURY PREVENTION

Volume: 8

Issue: 1

Pages: 34-37

DOI: 10.15171/jrip.2019.07

Published: MAR 2019

Impact Factor (2017): 000

Quartile in Category: 000

 


NO. (7)

Title: Optimizing adsorptive removal of malachite green and methyl orange dyes from simulated wastewater by Mn-doped CuO-Nanoparticles loaded on activated carbon using CCD-RSM: Mechanism, regeneration, isotherm, kinetic, and thermodynamic studies

 

Authors: Sharifpour, E (Sharifpour, Ebrahim); Dil, EA (Dil, Ebrahim Alipanahpour); Asfaram, A (Asfaram, Arash); Ghaedi, M (Ghaedi, Mehrorang); Goudarzi, A (Goudarzi, Alireza)

 

Abstract:
In the present work, Mn-doped CuO-NPs-AC was prepared by a simple method, characterized using various techniques such as FESEM, EDX, XRD, PSD, and pH(pzc) and finally used for the adsorption of malachite green (MG) and methyl orange (MO) in a number of single and binary solutions. A series of adsorption experiments were conducted to investigate and optimize the influence of various factors (such as different pH, concentration of MG and MO, adsorbent mass, and sonication time) on the simultaneous adsorption of MG and MO using response surface methodology. Under optimal conditions of pH 10, adsorbent dose of 0.02 g, MG concentration of 30 mg L-1, MO concentration of 30 mg L-1, and sonication time of 4.5 min at room temperature, the maximum predicted adsorption was observed to be 100.0%, for both MG and MO, showing that there is a favorable harmony between the experimental data and model predictions. The adsorption isotherm of MO and MG by Mn-doped CuO-NPs-AC could be well clarified by the Langmuir model with maximum adsorption capacity of 320.69 mg g(-1) and 290.11 mg g(-1) in the single solution and 233.02 mg g(-1) and 205.53 mg g(-1) in the binary solution by 0.005 g of adsorbent mass for MG and MO, respectively. Kinetic studies also revealed that both MG and MO adsorption were better defined by the pseudo-second order model for both solutions. In addition, the thermodynamic constant studies disclosed that the adsorption of MG and MO was likely to be influenced by a physisorption mechanism. Eventually, the reusability of the Mn-doped CuO-NPs-AC after six times showed a reduction in the adsorption percentage of MG and MO.

 

APPLIED ORGANOMETALLIC CHEMISTRY

Volume: 33

Issue: 3

Article Number: e4768

DOI: 10.1002/aoc.4768

Published: MAR 2019

Impact Factor (2017): 3.581

Quartile in Category: Q1

 


NO. (8)
Title: Metformin attenuates oxidative stress and liver damage after bile duct ligation in rats

 

Authors: Sadeghi, H (Sadeghi, Heibatollah); Jahanbazi, F (Jahanbazi, Fatemeh); Sadeghi, H (Sadeghi, Hossein); Omidifar, N (Omidifar, Navid); Alipoor, B (Alipoor, Behnam); Kokhdan, EP (Kokhdan, Esmaeel Panahi); Mousavipoor, SM (Mousavipoor, Seyed Mehdi); Mousavi-Fard, SH (Mousavi-Fard, Seyed Hossein); Doustimotlagh, AH (Doustimotlagh, Amir Hossein)

 

Abstract:
The aim of the current study was to investigate the antioxidative effect of metformin (MTF) on bile duct ligation (BDL)-induced hepatic disorder and histological damage in rats. The rats were divided into 4 groups including sham control (SC), BDL alone (BDL surgery), MTF1 (BDL surgery and administration of 250 mg/kg of MFM) and MTF2 (BDL surgery and administration of 500 mg/kg of MTF). After BDL, the animals treated with MTF by gavage for 10 days. Hematoxylin and eosin staining, biochemical analysis and oxidative stress markers were assayed to determine histological alterations, liver functions, and oxidant/antioxidant status. Hepatotoxicity was verified by remarkable increase in plasma levels of aminotransferases and alkaline phosphatase activity and liver histology 10 days after the BDL surgery. Our finding showed that treatment with MTF markedly reduced plasma alkaline phosphatase and alleviated liver injury indices (P <= 0.05). Furthermore, BDL caused a considerable increase in the protein carbonyl and malondialdehyde content (P <= 0.05). However, MTF reduces oxidative stress by constraining the protein oxidation and lipid peroxidation, and increases antioxidant reserve by increasing the ferric reducing ability of plasma and reducing glutathione levels. MTF exerts antioxidative effects in the liver fibrosis and may represent a hepato-protective effect when given to rats with BDL-induced hepatic injury.

 

RESEARCH IN PHARMACEUTICAL SCIENCES

Volume: 14

Issue: 2

Pages: 122-129

DOI: 10.4103/1735-5362.253359

Published: MAR-APR 2019

Impact Factor (2017): 1.587

Quartile in Category: Q1

 


NO. (9)

Title: Ultrasound combined with manganese-oxide nanoparticles loaded on activated carbon for extraction and pre-concentration of thymol and carvacrol in methanolic extracts of Thymus daenensis, Salvia officinalis, Stachys pilifera, Satureja khuzistanica, and mentha, and water samples

 

Authors: Asfaram, A (Asfaram, Arash); Sadeghi, H (Sadeghi, Hossein); Goudarzi, A (Goudarzi, Alireza); Kokhdan, EP (Kokhdan, Esmaeel Panahi); Salehpour, Z (Salehpour, Zeinab)

 

Abstract:
A dispersive micro solid-phase extraction (DMSPE) technique was developed using manganese-oxide nanoparticles loaded on activated carbon (Mn3O4-NPs-AC) as an effective sorbent combined with ultrasound for the extraction and determination of a trace amount of thymol and carvacrol in methanolic extracts of Thymus daenensis, Salvia officinalis, Stachys pilifera, Satureja khuzistanica and mentha, and water samples. Thymol and carvacrol phenolic compounds were extracted from real samples using acetonitrile (ACN) as the desorption solvent. Using central composite design (CCD), the effects of pH, ionic strength (NaCl), nano-sorbent mass, contact time, and desorption volume were investigated. Additionally, based on five-level variables, response surface methodology was used to determine the individual and interactive effects between factors on the process. The optimized extraction conditions included 12 mg of Mn3O4-NPs-AC as the sorbent, 300 mu L of ACN as the desorption solvent, pH 3.0, 0.5 w/v% of NaCl, and 4.5 min sonication time. Under the optimized conditions, for all the samples, the limits of detection were 0.054-0.104 ng mL(-1) and the limits of quantification were 0.178-0.345 ng mL(-1). The correlation coefficients of the calibration curves were > 0.985, i.e. in the range of 0.4-6000 ng mL(-1). To validate the effects of the matrix, the recovery, reproducibility, repeatability, and overall uncertainty were calculated for the five methanolic extracts, at 50, 100, and 500 ng mL(-1). The recovery ranged between 94.5% and 109.0% with a relative standard deviation of < 8.0% for the repeatability and reproducibility precision, which strongly supports the favorable repeatability and reproducibility of the method. The presented method also has the excellent sorbent features of NPs for the sorption of the analyte, which is due to the use of ultrasound for dispersion of the material in the sample matrix.

 

ANALYST

Volume: 144

Issue: 6

Pages: 1923-1934

DOI: 10.1039/c8an02338g

Published: MAR 21 2019

Impact Factor (2017): 3.864

Quartile in Category: Q1

 


NO. (10)
Title: Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM

 

Authors: By:Vahdani, SP (Vahdani, Shiva Pour); Khanzadeh, T (Khanzadeh, Taghi); Karimi, F (Karimi, Fattaneh); Esfahlan, AJ (Esfahlan, Aylin Jahanban); Ghavipanjeh, S (Ghavipanjeh, Somayeh); Ghasemi, A (Ghasemi, Amin); Heydarabad, MZ (Heydarabad, Milad Zadi); Azizi, H (Azizi, Hakim); Darbin, A (Darbin, Akbar); Khorrami, A (Khorrami, Arash); Azimi, A (Azimi, Ako)

 

Abstract:
Objectives: Acute lymphoblastic leukemia (ALL) is considered one of the common types of cancers in childhood with an incidence of up to 25%. In addition, drug resistance is a phenomenon which reduces the chances of overcoming cancer. Further, a phytoalexin combination called resveratrol can sensitize the leukemic cells to apoptotic cell death. Due to the importance of the above-mentioned issues, the present study aimed to evaluate the effect of resveratrol on BAX and BCL-2 expression levels and apoptosis induction.

Materials and Methods: CCRF-CEM cultured cells were treated by resveratrol at doses of 15, 50, and 100 mu M based on previous studies. Furthermore, RT Polymerase chain reaction (PCR) was conducted to assess the BAX and BCL-2 gene expression. Moreover, the amount of apoptosis induction was analyzed by annexin V staining method.

Results: Based on the results, time and concentration were found to play a critical role in resveratrol-induced apoptosis. Additionally, BAX upregulation and BCL-2 downregulation excreted by resveratrol in CCRF-CEM cells resulted in predisposing these cells to apoptosis.

Conclusions: In general, it was revealed that resveratrol could have a chemo-preventive activity by modifying the expression of BAX and BCL-2 genes. Finally, resveratrol was found to be a supplement drug in anti-leukemic therapy.

 

CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES

Volume: 6

Issue: 2

Pages: 209-213

Published: APR 2019

Impact Factor (2017): 000

Quartile in Category: 000

 


NO. (11)

Title: Bladder smooth muscle cell differentiation of the human induced pluripotent stem cells on electrospun Poly(lactide-co-glycolide) nanofibrous structure

 

Authors: Mirzaei, A (Mirzaei, Ali); Saburi, E (Saburi, Ehsan); Islami, M (Islami, Maryam); Ardeshirylajimi, A (Ardeshirylajimi, Abdolreza); Omrani, MD (Omrani, Mir Davood); Taheri, M (Taheri, Mohammad); Moghadam, AS (Moghadam, Abbas Shapouri); Ghafouri-Fard, S (Ghafouri-Fard, Soudeh)

 

Abstract:
Smooth muscle cell (SMC) regeneration plays an important role in retrieving the bladder-wall functionality and it can be achieved by a proper cell-co-polymer constructed by tissue engineering. Human induced pluripotent stem cells (iPSCs), which can be specifically prepared for the patient, was considered as cells in this study, and Poly(lactide-co-glycolide) (PLGA) as a most interesting polymer in biomedical applications was applied to the scaffold fabrication by electrospinning. After scaffold characterization, SMC differentiation potential of the human iPSCs was investigated while cultured on the PLGA nanofibrous scaffold by evaluation of the SMC related important gene and protein markers. Alpha-smooth muscle actin (ASMA), Smooth muscle 22 alpha (SM-22a) as two early SMC markers were significantly up regulated either two and three weeks after differentiation induction in human iPSCs cultured on PLGA compared to those cells cultured on the tissue culture polystyrene (TCPS). But Calponin-1, Caldesmon1 and myosin heavy chain (MHC) expression differences in human iPSCs cultured on PLGA and TCPS were significant only three weeks after differentiation induction based on its lately expression in the differentiation process. ASMA and MHC proteins were also considered for evaluation by immunocytochemistry on differentiated iPSCs whereas results showed higher expression of these proteins in stem cells grown on PLGA compared to the TCPS. According to the results, human iPSCs demonstrated a great SMC differentiation potential when grown on PLGA and it could be considered as a promising cell-co-polymer for use in bladder tissue engineering.

 

GENE

Volume: 694

Pages: 26-32

DOI: 10.1016/j.gene.2019.01.037

Published: APR 30 2019

Impact Factor (2017): 2.498

Quartile in Category: Q3


NO. (12)
Title: Efficient adsorption of Azure B onto CNTs/Zn:ZnO@Ni2P-NCs from aqueous solution in the presence of ultrasound wave based on multivariate optimization

 

Authors: Dil, EA (Dil, Ebrahim Alipanahpour); Ghaedi, M (Ghaedi, Mehrorang); Asfaram, A (Asfaram, Arash); Mehrabi, F (Mehrabi, Fatemeh); Sadeghfar, F (Sadeghfar, Fardin)

 

Abstract:

This work devoted to the investigation of adsorption Azure B (Az-B) onto CNTs/Zn:ZnO@Ni2P-NCs, while dependency of process efficiency to variables was optimized by the application response surface methodology. This adsorbent successfully using small amount 0.031 g is applicable to the adsorption of the high amount of Az-B in a short time. The optimum variables for achievement of maximum Az-B adsorption percentage (98.84%) were achieved using 0.031 g of CNTs/Zn:ZnO@Ni2P-NCs, 26.1 mgL-1.initial Az-B concentration, 8.2 min sonication time and pH 6.3. The kinetic of Az-B adsorption followed pseudo-second-order kinetic and equilibrium data strongly follow the Langmuir model.

 

Journal of Industrial and Engineering Chemistry

Volume: 74

Pages: 55-62

DOI: 10.1016/j.jiec.2018.12.050

Published: JUN 25 2019

Impact Factor (2017): 4.841

Quartile in Category: Q1

 


NO. (13)
Title: Antidiabetic Activity of Hydroalcoholic Extract of Myrtus communis (Myrtle) Fruits in Streptozotocin-Induced and Dexamethasone-Induced Diabetic Rats

 

Authors: Talebianpoor, Mohammad Sharif; Talebianpoor, Mohammad Shafee); Mansourian, Mahboubeh; Vafaiee-Nejad, Tahereh

 

Abstract: Background: Diabetes mellitus is characterized by an increase in blood glucose levels, resulting in insufficiency of insulin secretion, insulin resistance, or both. Objective: The effects of hydroalcoholic extract of the Myrtus communis (myrtle) fruits on Wistar rats were investigated in two types of diabetes mellitus. Materials and Methods: Intraperitoneal administration of streptozotocin (60 mg/kg) was used to induce Type I diabetes. Type II diabetes was induced by subcutaneous injection of 1 mg/kg/day dexamethasone for 10 days. Two groups of the diabetic animals received the hydroalcoholic extract of the fruit by gavage (250 mg/kg and 500 mg/kg for 45 and 10 days) and the diabetic control groups receiving distilled water. Results: The hydroalcoholic extract of M. communis fruits reduced the serum levels of the glucose, triglyceride, urine volume, urine protein, and malondialdehyde at the end of the 45 day. In Type II diabetic rats, there was a significant effect on plasma glucose levels. On this day, blood glucose-lowering effect was significantly observed after insulin administration as C2>D2M500>D2M250>D2 (P <= 0.05). The plasma level of insulin was completely reversed. There were no differences in the other biochemical parameters. Conclusions: The hydroalcoholic extract of M. communis fruits has a significant effect on the improvement of diabetes mellitus complications especially Type II diabetic animals, which begins with insulin resistance.

 

PHARMACOGNOSY RESEARCH

Volume: 11

 

Issue: 2

Pages: 115-120

DOI: 10.4103/pr.pr_160_18

Published:APR-JUN 2019

Document Type:Article

Impact Factor (2017): 1.730

Quartile in Category: Q3

 

 

 


NO. (14)
Title:
Magnetic dispersive micro-solid phase extraction with the CuO/ZnO@Fe3O4-CNTs nanocomposite sorbent for the rapid pre-concentration of chlorogenic acid in the medical extract of plants, food, and water samples

 

Authors: Ebrahim Alipanahpour DilArash Asfaram*  and  Fardin Sadeghfar 

 

Abstract: Herein, the CuO/ZnO@Fe3O4-carbon nanotubes (CNTs)-nanocomposite (NC), as a sorbent for magnetic dispersive micro-solid phase extraction, was developed for the determination of chlorogenic acid (CGA) in the medical extract of plants, food, and water samples in combination with high-performance liquid chromatography. The CuO/ZnO@Fe3O4-CNTs-NC was characterized by FESEM, EDS, XRD, TEM, BET, FT-IR, and VSM. Significant parameters (pH, temperature, eluent volume, vortex time, sonication time, CuO/ZnO@Fe3O4-CNTs-NC mass, and desorption time) that affected the extraction efficiency of CGA were optimized using Plackett-Burman as the screening design and the central composite design as the optimization strategy. Under the optimized conditions, the analytical parameters were obtained. The optimized method showed good linearity and a linear regression coefficient >0.9893. The enrichment factors ranged from 102.43 to 123.76 with the preconcentration factor of 60.0. The limits of detection (LOD) and the limits of quantification (LOQ) were 0.034-0.061 ng mL-1 and 0.114-0.202 ng mL-1, respectively. The method also reflected acceptable accuracy (from 94.07% to 109.7%) and the broad potential applications of CuO/ZnO@Fe3O4-CNTs-NC for the detection of CGA content in the medical extract of plants, food, and water samples.

 

ANALYST

Volume: 144

Issue: 6

Pages:  2684-2695

DOI: 10.1039/C8AN02484G

Published: APR 21 2019

Impact Factor (2017): 3.864

Quartile in Category: Q1

 


NO. (15)
Title: Impact of olive oil and honey on healing of diabetic foot: a randomized controlled trial

 

Authors: Karimi, Zohreh; Behnammoghadam, Mohammad; Rafiei, Hossein; Abdi, Naeem; Zoladl, Mohammad; Talebianpoor, Mohammad Shari; Arya, Arash; Khastavaneh, Maryam

 

Abstract:

Background: This study aimed 1) to examine the impact of honey on diabetic foot; 2) to examine the effect of olive oil on diabetic foot; and 3) to compare the impact of honey and olive oil in the healing of diabetic foot.

Methods: In this randomized controlled trial, 45 patients took part. Patients were randomly assigned to three groups. In the honey group, the wound was dressed using gauzes with honey daily for 1 month. In the olive oil group, the wound was dressed using gauzes with olive oil (4 mL) daily for 1 month. Patients in the control group received usual dressing. Wounds were assessed before and after intervention using the Wagner scoring system and the checklist of diabetic foot healing (where a higher score indicates better wound healing).

Results: Demographic characteristics of patients in the three groups were similar. Mean scores of tissue around the wound, wound grade, wound drainage, and wound healing were similar before intervention in all three groups. After intervention, means score of tissue around the wound, wound grade, wound drainage, and wound healing were significantly higher in patients in the honey and olive oil groups compared to patients in the control group.

Conclusion: The results of this study reveal that honey is as effective as olive oil in the treatment of diabetic foot. Given the few studies on this topic, further investigation is needed.

JOURNAL OF RENAL INJURY PREVENTION

Volume: 12

Issue: -

Pages:  34-37

DOI: 10.2147/CCID.S198577

Published: 2019

Impact Factor (2017): 2.8

Quartile in Category: Q1

 

 

 


NO. (16)
Title: Treatment of cancer stem cells from human colon adenocarcinoma cell line HT-29 with resveratrol and sulindac induced mesenchymal-endothelial transition rate

 

Authors: Pouyafar, Ayda; Rezabakhsh, Aysa; Rahbarghazi, Reza; Heydarabad, Milad Zadi; Shokrollahi, Elhameh; Sokullu, Emel; Khaksar, Majid; Nourazarian, Alireza; Avci, Cigir Biray 

 

Abstract:

In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in endothelial induction medium containing sulindac and resveratrol. Expression of endothelial markers such as the von Willebrand factor (vWF) and vascular endothelial cadherin (VE-cadherin) and genes participating in mesenchymal-to-epithelial transition was studied by real-time PCR assay. Protein levels of Wnt-3a and angiogenic factor YKL-40 were examined by western blotting. ELISA was used to determine the level of N-acetylgalactosaminyltransferase 11 (GALNT11) during mesenchymal-endothelial transition. Autophagy status was monitored by PCR array under treatment with the resveratrol plus sulindac. Results showed that resveratrol and sulindac had the potential to decrease the cell survival of HT-29 cancer cells and the clonogenic capacity of cancer stem cells compared with the control (p<0.05). The expression of VE-cadherin and vWF was induced in cancer stem cells incubated with endothelial differentiation medium enriched with resveratrol (p<0.05). Interestingly, the Wnt-3a level was increased in the presence of resveratrol and sulindac (p<0.05). YKL-40 was reduced after cell exposure to sulindac and resveratrol. The intracellular content of resistance factor GALNT11 was diminished after treatment with resveratrol (p<0.05). Resveratrol had the potential to induce the transcription of autophagy signaling genes in cancer stem cells during endothelial differentiation (p<0.05). These data show that resveratrol could increase cancer stem cell trans-differentiation toward endothelial lineage while decrease cell resistance by modulation of autophagy signaling and GALNT11 synthesis.

CELL AND TISSUE RESEARCH

Volume: 376

Issue: 3

Pages:  377-388

DOI: 10.1007/s00441-019-02998-9

Published: Jun 2019

Impact Factor (2017): 3.043

Quartile in Category: Q3

 


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